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CRYSTAL STRUCTURE OF THERMOACTINOMYCES VULGARIS R-47 ALPHA-AMYLASE 2 MUTANT F286A

Source organism: Thermoactinomyces vulgaris
Biochemical function: metal ion binding
Biological process: metabolic process
Cellular component: not assigned

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EC 3.2.1.135: Neopullulanase

Reaction catalysed:
Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
Systematic name:
Pullulan 4-D-glucanohydrolase (panose-forming)
Alternative Name(s):
  • Pullulanase II

GO terms

Sequence families

Pfam Protein families (Pfam)
PF16657
Domain description: Maltogenic Amylase, C-terminal domain
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of Pfam domain PF16657 (Maltogenic Amylase, C-terminal domain) in Neopullulanase 2. Showing 1 copy in chain A.

PF00128
Domain description: Alpha amylase, catalytic domain
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of Pfam domain PF00128 (Alpha amylase, catalytic domain) in Neopullulanase 2. Showing 1 copy in chain A.

PF02903
Domain description: Alpha amylase, N-terminal ig-like domain
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of Pfam domain PF02903 (Alpha amylase, N-terminal ig-like domain) in Neopullulanase 2. Showing 1 copy in chain A.

InterPro InterPro annotations
IPR006047
Domain description: Glycosyl hydrolase, family 13, catalytic domain
Occurring in:
  1. Neopullulanase 2
IPR004185
Domain description: Glycoside hydrolase, family 13, N-terminal Ig-like domain
Occurring in:
  1. Neopullulanase 2
IPR032091
Domain description: Maltogenic Amylase, C-terminal
Occurring in:
  1. Neopullulanase 2
IPR013780
Domain description: Glycosyl hydrolase, all-beta
Occurring in:
  1. Neopullulanase 2
IPR045857
Domain description: Oligo-1,6-glucosidase, domain 2
Occurring in:
  1. Neopullulanase 2
IPR017853
Domain description: Glycoside hydrolase superfamily
Occurring in:
  1. Neopullulanase 2
IPR014756
Domain description: Immunoglobulin E-set
Occurring in:
  1. Neopullulanase 2
IPR013783
Domain description: Immunoglobulin-like fold
Occurring in:
  1. Neopullulanase 2

Structure domains

CATH CATH domains
3.20.20.80
Class: Alpha Beta
Architecture: Alpha-Beta Barrel
Topology: TIM Barrel
Homology: Glycosidases
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of CATH domain 3.20.20.80 (TIM Barrel) in Neopullulanase 2. Showing 1 copy in chain A.
2.60.40.1180
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: Golgi alpha-mannosidase II
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of CATH domain 2.60.40.1180 (Immunoglobulin-like) in Neopullulanase 2. Showing 1 copy in chain A.
3.90.400.10
Class: Alpha Beta
Architecture: Alpha-Beta Complex
Topology: Oligo-1,6-glucosidase; domain 2
Homology: Oligo-1,6-glucosidase; Domain 2
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of CATH domain 3.90.400.10 (Oligo-1,6-glucosidase; domain 2) in Neopullulanase 2. Showing 1 copy in chain A.
2.60.40.10
Class: Mainly Beta
Architecture: Sandwich
Topology: Immunoglobulin-like
Homology: Immunoglobulins
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of CATH domain 2.60.40.10 (Immunoglobulin-like) in Neopullulanase 2. Showing 1 copy in chain A.
SCOP SCOP annotations
51012
Class: All beta proteins
Fold: Glycosyl hydrolase domain
Superfamily: Glycosyl hydrolase domain
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of SCOP domain 51012 (alpha-Amylases, C-terminal beta-sheet domain) in Neopullulanase 2. Showing 1 copy in chain A.
51446
Class: Alpha and beta proteins (a/b)
Fold: TIM beta/alpha-barrel
Superfamily: (Trans)glycosidases
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of SCOP domain 51446 (Amylase, catalytic domain) in Neopullulanase 2. Showing 1 copy in chain A.
81282
Class: All beta proteins
Fold: Immunoglobulin-like beta-sandwich
Superfamily: E set domains
Occurring in:
  1. Neopullulanase 2
The deposited structure of PDB entry 1jf5 contains 2 copies of SCOP domain 81282 (E-set domains of sugar-utilizing enzymes) in Neopullulanase 2. Showing 1 copy in chain A.

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