› 1jds

X-ray diffraction
1.8Å resolution

5'-DEOXY-5'-METHYLTHIOADENOSINE PHOSPHORYLASE COMPLEX WITH PHOSPHATE (SPACE GROUP P21)

Released:
DOI: 10.2210/pdb1jds/pdb
PDB entry 1jds coloured by chain, front view.
PDB entry 1jds coloured by chain, side view.
PDB entry 1jds coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Three-dimensional structure of a hyperthermophilic 5'-deoxy-5'-methylthioadenosine phosphorylase from Sulfolobus solfataricus.
Appleby TC, Mathews II, Porcelli M, Cacciapuoti G, Ealick SE
J Biol Chem 276 39232-42 (2001)
PMID: 11489901

Function and Biology Details

Reactions catalysed: 
Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156172 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Purine nucleoside phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 236 amino acids
Theoretical weight: 25.76 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P50389 (Residues: 1-236; Coverage: 100%)
Gene name: SSO2706
Sequence domains: Phosphorylase superfamily
Structure domains: Nucleoside phosphorylase domain

Ligands and Environments

2 bound ligands:
Ligand PO4 6 x PO4
Ligand TRS 6 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P21
Unit cell:
a: 101.5Å b: 86.2Å c: 102.1Å
α: 90° β: 119.2° γ: 90°
R-values:
R R work R free
0.22 0.22 0.249
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Methylthioadenosine/S-adenosylhomocysteine nucleosidase, a critical enzyme for bacterial metabolism.
Parveen et al. (2011)
5 more

1 mention without citation

Discovery of a thermophilic protein complex stabilized by topologically interlinked chains.
Boutz et al. (2007)