› 1jbm

X-ray diffraction
1.85Å resolution

Heptameric crystal structure of Mth649, an Sm-like archaeal protein from Methanobacterium thermautotrophicum

Released:
DOI: 10.2210/pdb1jbm/pdb
PDB entry 1jbm coloured by chain, front view.
PDB entry 1jbm coloured by chain, side view.
PDB entry 1jbm coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
The oligomerization and ligand-binding properties of Sm-like archaeal proteins (SmAPs).
Mura C, Kozhukhovsky A, Gingery M, Phillips M, Eisenberg D
Protein Sci 12 832-47 (2003)
PMID: 12649441

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127850 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative snRNP Sm-like protein Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 86 amino acids
Theoretical weight: 9.57 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O26745 (Residues: 1-81; Coverage: 100%)
Gene name: MTH_649
Sequence domains: LSM domain
Structure domains: SH3 type barrels.

Ligands and Environments

2 bound ligands:
Ligand ACY 5 x ACY
Ligand EDO 11 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P1
Unit cell:
a: 45.066Å b: 54.077Å c: 62.346Å
α: 87.58° β: 72.86° γ: 81.45°
R-values:
R R work R free
0.196 0.196 0.238
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Hfq structure, function and ligand binding.
Brennan et al. (2007)
6 more

3 mentions without citation

Protein complex compositions predicted by structural similarity.
Davis et al. (2006)
2 more