› 1jb1

X-ray diffraction
2.8Å resolution

Lactobacillus casei HprK/P Bound to Phosphate

Released:
DOI: 10.2210/pdb1jb1/pdb
PDB entry 1jb1 coloured by chain, front view.
PDB entry 1jb1 coloured by chain, side view.
PDB entry 1jb1 coloured by chain, top view.
Source organism: Lacticaseibacillus casei
Primary publication:
X-ray structure of HPr kinase: a bacterial protein kinase with a P-loop nucleotide-binding domain.
Fieulaine S, Morera S, Poncet S, Monedero V, Gueguen-Chaignon V, Galinier A, Janin J, Deutscher J, Nessler S
EMBO J 20 3917-27 (2001)
PMID: 11483495

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193152 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HPr kinase/phosphorylase Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.96 KDa
Source organism: Lacticaseibacillus casei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RE09 (Residues: 128-319; Coverage: 60%)
Gene name: hprK
Sequence domains: HPr Serine kinase C-terminal domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand PO4 1 x PO4
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6322
Unit cell:
a: 107.738Å b: 107.738Å c: 66.498Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.235 0.288
Expression system: Escherichia coli

Quick links

Citations

15 review citations

How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.
Deutscher et al. (2006)
14 more

8 mentions without citation

X-ray structure of HPr kinase: a bacterial protein kinase with a P-loop nucleotide-binding domain.
Fieulaine et al. (2001)
7 more