› 1jaz

X-ray diffraction
2.27Å resolution

Crystal Structure of Monoclinic Form of D90E Mutant of Escherichia coli Asparaginase II

Released:
DOI: 10.2210/pdb1jaz/pdb
PDB entry 1jaz coloured by chain, front view.
PDB entry 1jaz coloured by chain, side view.
PDB entry 1jaz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site.
Borek D, Kozak M, Pei J, Jaskolski M
FEBS J 281 4097-111 (2014)
PMID: 25040257

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133590 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparaginase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 326 amino acids
Theoretical weight: 34.64 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P00805 (Residues: 23-348; Coverage: 100%)
Gene names: JW2924, ansB, b2957
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: C2
Unit cell:
a: 73.118Å b: 133.076Å c: 62.565Å
α: 90° β: 108.78° γ: 90°
R-values:
R R work R free
0.183 0.18 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Therapeutic l-asparaginase: upstream, downstream and beyond.
Lopes et al. (2017)
4 more

3 mentions without citation

Crystal structure of active site mutant of antileukemic L-asparaginase reveals conserved zinc-binding site.
Borek et al. (2014)
2 more