1j8h

X-ray diffraction
2.4Å resolution

Crystal Structure of a Complex of a Human alpha/beta-T cell Receptor, Influenza HA Antigen Peptide, and MHC Class II Molecule, HLA-DR4

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
PDBe Complex ID:
PDB-CPX-134724 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
HLA class II histocompatibility antigen, DR alpha chain Chain: A
Molecule details ›
Chain: A
Length: 181 amino acids
Theoretical weight: 21.08 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01903 (Residues: 26-206; Coverage: 79%)
Gene names: HLA-DRA, HLA-DRA1
Sequence domains:
Structure domains:
HLA class II histocompatibility antigen, DRB1 beta chain Chain: B
Molecule details ›
Chain: B
Length: 192 amino acids
Theoretical weight: 22.51 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: P01911 (Residues: 30-221; Coverage: 81%)
Gene name: HLA-DRB1
Sequence domains:
Structure domains:
Hemagglutinin HA1 chain Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.51 KDa
Source organism: Alphainfluenzavirus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03437 (Residues: 322-334; Coverage: 2%)
Gene name: HA
T cell receptor alpha chain constant Chain: D
Molecule details ›
Chain: D
Length: 212 amino acids
Theoretical weight: 23.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01848 (Residues: 1-97; Coverage: 69%)
Gene names: TCRA, TRAC
Sequence domains: Domain of unknown function (DUF1968)
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: E
Molecule details ›
Chain: E
Length: 246 amino acids
Theoretical weight: 28.04 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01850 (Residues: 1-133; Coverage: 76%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, NDG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-C
Spacegroup: C2
Unit cell:
a: 143.753Å b: 73.317Å c: 123.033Å
α: 90° β: 108.52° γ: 90°
R-values:
R R work R free
0.211 0.211 0.246
Expression systems:
  • Drosophila melanogaster
  • Escherichia coli BL21(DE3)