› 1j77

X-ray diffraction
1.5Å resolution

Crystal Structure of Gram-negative Bacterial Heme Oxygenase Complexed with Heme

Released:
DOI: 10.2210/pdb1j77/pdb
PDB entry 1j77 coloured by chain, front view.
PDB entry 1j77 coloured by chain, side view.
PDB entry 1j77 coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of heme oxygenase from the gram-negative pathogen Neisseria meningitidis and a comparison with mammalian heme oxygenase-1.
Schuller DJ, Zhu W, Stojiljkovic I, Wilks A, Poulos TL
Biochemistry 40 11552-8 (2001)
PMID: 11560504

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HemO Chain: A
Molecule details ›
Chain: A
Length: 209 amino acids
Theoretical weight: 23.61 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9RGD9 (Residues: 22-230; Coverage: 91%)
Gene names: NMA510612_2169, hemO
Sequence domains: Heme oxygenase
Structure domains: Heme oxygenase-like

Ligands and Environments

1 bound ligand:
Ligand HEM 1 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: P43212
Unit cell:
a: 63.167Å b: 63.167Å c: 100.378Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.229 0.227 0.26
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

12 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
11 more

3 mentions without citation

Ruffling of metalloporphyrins bound to IsdG and IsdI, two heme-degrading enzymes in Staphylococcus aureus.
Lee et al. (2008)
2 more