› 1j49

X-ray diffraction
2.2Å resolution

INSIGHTS INTO DOMAIN CLOSURE, SUBSTRATE SPECIFICITY AND CATALYSIS OF D-LACTATE DEHYDROGENASE FROM LACTOBACILLUS BULGARICUS

Released:
DOI: 10.2210/pdb1j49/pdb
PDB entry 1j49 coloured by chain, front view.
PDB entry 1j49 coloured by chain, side view.
PDB entry 1j49 coloured by chain, top view.
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Primary publication:
Domain closure, substrate specificity and catalysis of D-lactate dehydrogenase from Lactobacillus bulgaricus.
Razeto A, Kochhar S, Hottinger H, Dauter M, Wilson KS, Lamzin VS
J Mol Biol 318 109-19 (2002)
PMID: 12054772

Function and Biology Details

Reaction catalysed: 
(R)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-lactate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 333 amino acids
Theoretical weight: 37.12 KDa
Source organism: Lactobacillus delbrueckii subsp. bulgaricus
Expression system: Escherichia coli
UniProt:
  • Canonical: P26297 (Residues: 1-333; Coverage: 100%)
Gene names: Ldb0101, ldhA
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 79.4Å b: 79.4Å c: 228.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.207 0.271
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Evolution of rosmarinic acid biosynthesis.
Petersen et al. (2009)
2 more

8 mentions without citation

Development of a High-Throughput, In Vivo Selection Platform for NADPH-Dependent Reactions Based on Redox Balance Principles.
Zhang et al. (2018)
7 more