› 1iyp

X-ray diffraction
2Å resolution

Toho-1 beta-Lactamase In Complex With Cephalothin

Released:
DOI: 10.2210/pdb1iyp/pdb
PDB entry 1iyp coloured by chain, front view.
PDB entry 1iyp coloured by chain, side view.
PDB entry 1iyp coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Acyl-intermediate structures of the extended-spectrum class A beta-lactamase, Toho-1, in complex with cefotaxime, cephalothin, and benzylpenicillin.
Shimamura T, Ibuka A, Fushinobu S, Wakagi T, Ishiguro M, Ishii Y, Matsuzawa H
J Biol Chem 277 46601-8 (2002)
PMID: 12221102

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Toho-1 Chain: A
Molecule details ›
Chain: A
Length: 262 amino acids
Theoretical weight: 28.2 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: Q47066 (Residues: 30-291; Coverage: 100%)
Gene name: bla
Sequence domains:
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand CEP 1 x CEP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P3221
Unit cell:
a: 73.229Å b: 73.229Å c: 100.229Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.199 0.199 0.245
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Growing group of extended-spectrum beta-lactamases: the CTX-M enzymes.
Bonnet R. (2004)
10 more

1 mention without citation

Crystal structure of the Mycobacterium fortuitum class A beta-lactamase: structural basis for broad substrate specificity.
Sauvage et al. (2006)