› 1io9

X-ray diffraction
2.05Å resolution

THERMOPHILIC CYTOCHROME P450 (CYP119) FROM SULFOLOBUS SOLFATARICUS: HIGH RESOLUTION STRUCTURAL ORIGIN OF ITS THERMOSTABILITY AND FUNCTIONAL PROPERTIES

Released:
DOI: 10.2210/pdb1io9/pdb
PDB entry 1io9 coloured by chain, front view.
PDB entry 1io9 coloured by chain, side view.
PDB entry 1io9 coloured by chain, top view.
Source organism: Saccharolobus solfataricus
Primary publication:
Thermophilic cytochrome P450 (CYP119) from Sulfolobus solfataricus: high resolution structure and functional properties.
Park SY, Yamane K, Adachi S, Shiro Y, Weiss KE, Maves SA, Sligar SG
J Inorg Biochem 91 491-501 (2002)
PMID: 12237217

Function and Biology Details

Reaction catalysed: 
2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-176263 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 119 Chains: A, B
Molecule details ›
Chains: A, B
Length: 368 amino acids
Theoretical weight: 42.92 KDa
Source organism: Saccharolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q55080 (Residues: 1-368; Coverage: 100%)
Gene names: Saci_2081, cyp119
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44B2
Spacegroup: P43212
Unit cell:
a: 87.587Å b: 87.587Å c: 223.151Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.284
Expression system: Escherichia coli

Quick links

Citations

15 review citations

Hydrocarbon hydroxylation by cytochrome P450 enzymes.
Ortiz de Montellano PR. (2010)
14 more

5 mentions without citation

Two-dimensional NMR and all-atom molecular dynamics of cytochrome P450 CYP119 reveal hidden conformational substates.
Lampe et al. (2010)
4 more