1io4 Summary

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CRYSTAL STRUCTURE OF RUNX-1/AML1/CBFALPHA RUNT DOMAIN-CBFBETA CORE DOMAIN HETERODIMER AND C/EBPBETA BZIP HOMODIMER BOUND TO A DNA FRAGMENT FROM THE CSF-1R PROMOTER

The structure was published by Tahirov, T.H., Inoue-Bungo, T., Morii, H., et al., Yamamoto, M., Ishii, S., and Ogata, K., in 2001 in a paper entitled "Structural analyses of DNA recognition by the AML1/Runx-1 Runt domain and its allosteric control by CBFbeta." (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 3.0 Å and deposited in 2001.

The experimental data on which the structure is based was also deposited.

This entry contains 12 copies of 5 unique biopolymers (complete list).

It also contains one or more heterogenic compounds (e.g., ligands, co-factors, ions, modified amino acids, etc.); see here for a complete list.

The molecule most likely forms heterohexamers.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CAAT/ENHANCER BINDING PROTEIN BETA P17676 (259-336) (CEBPB_HUMAN)search Homo sapienssearch < 90% 78 89%
B CAAT/ENHANCER BINDING PROTEIN BETA P17676 (259-336) (CEBPB_HUMAN)search Homo sapienssearch < 90% 78 89%
C RUNT-RELATED TRANSCRIPTION FACTOR 1 Q03347 (60-182) (RUNX1_MOUSE)search Mus musculussearch < 90% 123 97%
D CORE-BINDING FACTOR, BETA SUBUNIT Q08024 (1-141) (PEBB_MOUSE)search Mus musculussearch < 90% 141 90%


This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P17676 (259 - 336) CAAT/ENHANCER BINDING PROTEIN BETA Homo sapiens
Q03347 (60 - 182) RUNT-RELATED TRANSCRIPTION FACTOR 1 Mus musculus
Q08024 (1 - 141) CORE-BINDING FACTOR, BETA SUBUNIT Mus musculus

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, B Leucine zipper domainsearch Single alpha-helices involved in coiled-coils or other helix-helix interfacessearch Basic region leucine zippersearch
C RUNT domainsearch Immunoglobulin-likesearch Runt domainsearch
D Core binding factor beta, CBFsearch Polyomavirus Enhancer Binding Protein 2; Chain: A;search Core binding factor beta subunitsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B (P17676) sequence-specific DNA bindingsearch sequence-specific DNA binding transcription factor activitysearch regulation of transcription, DNA-templatedsearch
C (Q03347) sequence-specific DNA binding transcription factor activitysearch DNA bindingsearch ATP bindingsearch regulation of transcription, DNA-templatedsearch nucleussearch
D (Q08024) transcription coactivator activitysearch nucleussearch

Chain InterPro annotation
A, B Basic-leucine zipper domainsearch
C Acute myeloid leukemia 1 protein (AML1)/Runtsearch p53-like transcription factor, DNA-bindingsearch p53/RUNT-type transcription factor, DNA-binding domainsearch Runt domainsearch
D Core binding factor, beta subunitsearch