› 1ik3

X-ray diffraction
2Å resolution

LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 13(S)-HYDROPEROXY-9(Z),11(E)-OCTADECADIENOIC ACID

Released:
DOI: 10.2210/pdb1ik3/pdb
PDB entry 1ik3 coloured by chain, front view.
PDB entry 1ik3 coloured by chain, side view.
PDB entry 1ik3 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Three-dimensional structure of a purple lipoxygenase.
Skrzypczak-Jankun E, Bross RA, Carroll RT, Dunham WR, Funk MO
J Am Chem Soc 123 10814-20 (2001)
PMID: 11686682

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 9S-lipoxygenase-3 Chain: A
Molecule details ›
Chain: A
Length: 857 amino acids
Theoretical weight: 96.92 KDa
Source organism: Glycine max
UniProt:
  • Canonical: P09186 (Residues: 1-857; Coverage: 100%)
Gene names: LOX1.3, LOX3
Sequence domains:
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand FE 1 x FE
Ligand 13S 1 x 13S
Ligand 13R 1 x 13R
Ligand 9OH 1 x 9OH
Ligand 11O 1 x 11O
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 112.65Å b: 137.28Å c: 61.89Å
α: 90° β: 95.56° γ: 90°
R-values:
R R work R free
0.261 0.196 0.296

Quick links

Citations

5 review citations

Lipoxygenases: occurrence, functions and catalysis.
Liavonchanka et al. (2006)
4 more

17 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
16 more