› 1iir

X-ray diffraction
1.8Å resolution

Crystal Structure of UDP-glucosyltransferase GtfB

Released:
DOI: 10.2210/pdb1iir/pdb
PDB entry 1iir coloured by chain, front view.
PDB entry 1iir coloured by chain, side view.
PDB entry 1iir coloured by chain, top view.
Source organism: Amycolatopsis orientalis
Primary publication:
Structure of the UDP-glucosyltransferase GtfB that modifies the heptapeptide aglycone in the biosynthesis of vancomycin group antibiotics.
Mulichak AM, Losey HC, Walsh CT, Garavito RM
Structure 9 547-57 (2001)
PMID: 11470430

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + vancomycin aglycone = UDP + devancoaminyl-vancomycin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161224 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Vancomycin aglycone glucosyltransferase Chain: A
Molecule details ›
Chain: A
Length: 415 amino acids
Theoretical weight: 43.78 KDa
Source organism: Amycolatopsis orientalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P96559 (Residues: 1-407; Coverage: 100%)
Gene name: gtfB
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:
Ligand SO4 1 x SO4
Ligand MG 2 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P41212
Unit cell:
a: 102.11Å b: 102.11Å c: 83.35Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.231
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

35 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
34 more

12 mentions without citation

Chapter 2: Biogenesis of the cell wall and other glycoconjugates of Mycobacterium tuberculosis.
Kaur et al. (2009)
11 more