› 1ig8

X-ray diffraction
2.2Å resolution

Crystal Structure of Yeast Hexokinase PII with the correct amino acid sequence

Released:
DOI: 10.2210/pdb1ig8/pdb
PDB entry 1ig8 coloured by chain, front view.
PDB entry 1ig8 coloured by chain, side view.
PDB entry 1ig8 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The high resolution crystal structure of yeast hexokinase PII with the correct primary sequence provides new insights into its mechanism of action.
Kuser PR, Krauchenco S, Antunes OA, Polikarpov I
J Biol Chem 275 20814-21 (2000)
PMID: 10749890

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138189 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-2 Chain: A
Molecule details ›
Chain: A
Length: 486 amino acids
Theoretical weight: 54 KDa
Source organism: Saccharomyces cerevisiae
UniProt:
  • Canonical: P04807 (Residues: 1-486; Coverage: 100%)
Gene names: HEX1, HKB, HXK2, NRB486, YGL253W
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE D03B-MX1
Spacegroup: I4
Unit cell:
a: 142.809Å b: 142.809Å c: 58.462Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.162 0.246

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Citations

5 review citations

Biochemistry and genetics of starch synthesis.
Keeling et al. (2010)
4 more

18 mentions without citation

Sequence and structural analyses of nuclear export signals in the NESdb database.
Xu et al. (2012)
17 more