› 1ici

X-ray diffraction
2.1Å resolution

CRYSTAL STRUCTURE OF A SIR2 HOMOLOG-NAD COMPLEX

Released:
DOI: 10.2210/pdb1ici/pdb
PDB entry 1ici coloured by chain, front view.
PDB entry 1ici coloured by chain, side view.
PDB entry 1ici coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Crystal structure of a SIR2 homolog-NAD complex.
Min J, Landry J, Sternglanz R, Xu RM
Cell 105 269-79 (2001)
PMID: 11336676

Function and Biology Details

Reaction catalysed: 
(1a) [protein]-N(6)-acetyl-L-lysine + NAD(+) = [protein]-N(6)-(1,1-(5-adenosylyl-alpha-D-ribose-1,2-di-O-yl)ethyl)-L-lysine + nicotinamide
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128044 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
NAD-dependent protein deacylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 256 amino acids
Theoretical weight: 28.44 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O28597 (Residues: 1-245; Coverage: 100%)
Gene names: AF_1676, cobB1
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ZN 2 x ZN
Ligand NAD 2 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2
Unit cell:
a: 65.41Å b: 94.54Å c: 93.78Å
α: 90° β: 95.29° γ: 90°
R-values:
R R work R free
0.203 0.199 0.249
Expression system: Escherichia coli

Quick links

Citations

66 review citations

The Sir2 family of protein deacetylases.
Blander et al. (2004)
65 more

15 mentions without citation

Erasers of histone acetylation: the histone deacetylase enzymes.
Seto et al. (2014)
14 more