› 1iaj

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE ATYPICAL PROTEIN KINASE DOMAIN OF A TRP CA-CHANNEL, CHAK (APO)

Released:
DOI: 10.2210/pdb1iaj/pdb
PDB entry 1iaj coloured by chain, front view.
PDB entry 1iaj coloured by chain, side view.
PDB entry 1iaj coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity.
Yamaguchi H, Matsushita M, Nairn AC, Kuriyan J
Mol Cell 7 1047-57 (2001)
PMID: 11389851

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transient receptor potential cation channel subfamily M member 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 32.25 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q923J1 (Residues: 1549-1828; Coverage: 15%)
Gene names: Chak, Ltrpc7, Trpm7
Sequence domains: Alpha-kinase family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 108.27Å b: 138.2Å c: 113.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.229 0.292
Expression system: Spodoptera frugiperda

Quick links

Citations

68 review citations

TRP channels as cellular sensors.
Clapham DE. (2003)
67 more

6 mentions without citation

eEF2K Inhibitor Design: The Progression of Exemplary Structure-Based Drug Design.
Klupt et al. (2023)
5 more