› 1i9b

X-ray diffraction
2.7Å resolution

X-RAY STRUCTURE OF ACETYLCHOLINE BINDING PROTEIN (ACHBP)

Released:
DOI: 10.2210/pdb1i9b/pdb
PDB entry 1i9b coloured by chain, front view.
PDB entry 1i9b coloured by chain, side view.
PDB entry 1i9b coloured by chain, top view.
Source organism: Lymnaea stagnalis
Primary publication:
Crystal structure of an ACh-binding protein reveals the ligand-binding domain of nicotinic receptors.
Brejc K, van Dijk WJ, Klaassen RV, Schuurmans M, van Der Oost J, Smit AB, Sixma TK
Nature 411 269-76 (2001)
PMID: 11357122

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo pentamer (preferred)
homo decamer
Assembly name:
PDBe Complex ID:
PDB-CPX-157653 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Acetylcholine-binding protein Chains: A, B, C, D, E
Molecule details ›
Chains: A, B, C, D, E
Length: 217 amino acids
Theoretical weight: 24.69 KDa
Source organism: Lymnaea stagnalis
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P58154 (Residues: 14-229; Coverage: 100%)
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains: Neurotransmitter-gated ion-channel ligand-binding domain

Ligands and Environments

2 bound ligands:
Ligand CA 8 x CA
Ligand EPE 5 x EPE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P42212
Unit cell:
a: 141.66Å b: 141.66Å c: 120.87Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.26 0.26 0.297
Expression system: Komagataella pastoris

Quick links

Citations

222 review citations

Emerging structure of the nicotinic acetylcholine receptors.
Karlin A. (2002)
221 more

68 mentions without citation

Mammalian nicotinic acetylcholine receptors: from structure to function.
Albuquerque et al. (2009)
67 more