Function and Biology Details
Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
- not assigned
Biological process:
- not assigned
Cellular component:
- not assigned
Sequence domains:
Structure domain:
Structure analysis Details
Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
E3 ubiquitin-protein ligase NEDD4
Molecule details ›
Chain: W
Length: 50 amino acids
Theoretical weight: 5.67 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Length: 50 amino acids
Theoretical weight: 5.67 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
Q62940 (Residues: 451-499; Coverage: 6%)
Sequence domains: WW domain
Structure domains: Ubiquitin Ligase Nedd4; Chain: W;
Amiloride-sensitive sodium channel subunit beta
Molecule details ›
Chain: B
Length: 17 amino acids
Theoretical weight: 1.73 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Length: 17 amino acids
Theoretical weight: 1.73 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: P37090 (Residues: 606-621; Coverage: 3%)
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
Refinement method:
torsion angle dynamics
Expression system: Escherichia coli BL21(DE3)
