› 1i47

X-ray diffraction
2.1Å resolution

MOLECULAR AND CRYSTAL STRUCTURE OF D(CGCGAATT(MO4)CGCG): THE WATSON-CRICK TYPE AND WOBBLE N4-METHOXYCYTIDINE/GUANOSINE BASE PAIRS IN B-DNA

Released:
DOI: 10.2210/pdb1i47/pdb
PDB entry 1i47 coloured by chain, front view.
PDB entry 1i47 coloured by chain, side view.
PDB entry 1i47 coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Crystallographic studies on damaged DNAs: III. N(4)-methoxycytosine can form both Watson-Crick type and wobbled base pairs in a B-form duplex.
Hossain MT, Chatake T, Hikima T, Tsunoda M, Sunami T, Ueno Y, Matsuda A, Takénaka A
J Biochem 130 9-12 (2001)
PMID: 11432773

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113941 (preferred)
Entry contents:
1 distinct DNA molecule
Macromolecule:
5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*(C45)P*GP*CP*GP)-3' Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.69 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 1 x MG
1 modified residue:
Ligand C45 2 x C45

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6B
Spacegroup: P212121
Unit cell:
a: 25.3Å b: 41.4Å c: 64.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.255
Expression system: Not provided

Quick links

Citations

5 citation in other articles

Crystal structures of DNA:DNA and DNA:RNA duplexes containing 5-(N-aminohexyl)carbamoyl-modified uracils reveal the basis for properties as antigene and antisense molecules.
Juan et al. (2007)
4 more