› 1i29

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF CSDB COMPLEXED WITH L-PROPARGYLGLYCINE

Released:
DOI: 10.2210/pdb1i29/pdb
PDB entry 1i29 coloured by chain, front view.
PDB entry 1i29 coloured by chain, side view.
PDB entry 1i29 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Structure of external aldimine of Escherichia coli CsdB, an IscS/NifS homolog: implications for its specificity toward selenocysteine.
Mihara H, Fujii T, Kato S, Kurihara T, Hata Y, Esaki N
J Biochem 131 679-85 (2002)
PMID: 11983074

Function and Biology Details

Reactions catalysed: 
(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine
L-selenocysteine + reduced acceptor = selenide + L-alanine + acceptor
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160167 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cysteine desulfurase Chain: A
Molecule details ›
Chain: A
Length: 406 amino acids
Theoretical weight: 44.48 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P77444 (Residues: 1-406; Coverage: 100%)
Gene names: JW1670, b1680, csdB, sufS, ynhB
Sequence domains: Aminotransferase class-V
Structure domains:

Ligands and Environments


Cofactor: Ligand PLP 1 x PLP
1 bound ligand:
Ligand LPG 1 x LPG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P43212
Unit cell:
a: 128.459Å b: 128.459Å c: 136.83Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.209 0.209 0.233
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Structure, function, and formation of biological iron-sulfur clusters.
Johnson et al. (2005)
9 more

13 mentions without citation

Chemical Biology of H2S Signaling through Persulfidation.
Filipovic et al. (2018)
12 more