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EBI PDBe PDBeView

PDBe Entry: 1i0l view

ANALYSIS OF AN INVARIANT ASPARTIC ACID IN HPRTS-ASPARAGINE MUTANT
Summary
Header TRANSFERASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 1.72 Å, R-factor: 25.0%, Free R-factor: 26.7%, Spacegroup: P 1 21 1
Released 29/05/2002, deposition: 29/01/2001, last revision: 24/02/2009
Authors Canyuk, B.search; Focia, P.J.search; Eakin, A.E.search
Primary citation The role for an invariant aspartic acid in hypoxanthine phosphoribosyltransferases is examined using saturation mutagenesis, functional analysis, and X-ray crystallography.
BIOCHEMISTRYsearch vol:40, pag:2754-2765 (2001) [PubMed ID 11258886 ]search
Keywords PHOSPHORIBOSYLTRANSFERASEsearch, NUCLEOTIDE METABOLISMsearch, PURINE SALVAGEsearch, TERNARY COMPLEXsearch, CATALYTIC BASEsearch
EC 2.4.2.8 ExPASy BRENDA search (A B)
Organism Trypanosoma cruzi 5693search(A B)
UniProt Hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) (Hypoxanthine-guanine phosphoribosyltransferase, putative) Q27796search (A B)
Solvent A, B
Related entries 1tc2, 1tc1, 1i0i, 1i13, 1i14
Polymers
Id Name Type UniProt Residues Observed
A, B HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE Protein Q27796 (Q27796_TRYCR)search
 221 87%
Heterogens
Id Name Ligands
A, B MAGNESIUM ION MG search
A, B 7-HYDROXY-PYRAZOLO[4,3-D]PYRIMIDINE 7HP search
A, B ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC ACID PRP search
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