› 1hy3

X-ray diffraction
1.8Å resolution

CRYSTAL STRUCTURE OF HUMAN ESTROGEN SULFOTRANSFERASE V269E MUTANT IN THE PRESENCE OF PAPS

Released:
DOI: 10.2210/pdb1hy3/pdb
PDB entry 1hy3 coloured by chain, front view.
PDB entry 1hy3 coloured by chain, side view.
PDB entry 1hy3 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the human estrogen sulfotransferase-PAPS complex: evidence for catalytic role of Ser137 in the sulfuryl transfer reaction.
Pedersen LC, Petrotchenko E, Shevtsov S, Negishi M
J Biol Chem 277 17928-32 (2002)
PMID: 11884392

Function and Biology Details

Reaction catalysed: 
3'-phosphoadenylyl sulfate + estrone = adenosine 3',5'-bisphosphate + estrone 3-sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156072 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfotransferase 1E1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 294 amino acids
Theoretical weight: 35.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49888 (Residues: 1-294; Coverage: 100%)
Gene names: STE, SULT1E1
Sequence domains: Sulfotransferase domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
Ligand PPS 2 x PPS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21
Unit cell:
a: 61.925Å b: 97.263Å c: 62.733Å
α: 90° β: 91.93° γ: 90°
R-values:
R R work R free
0.198 0.197 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Human sulfotransferases and their role in chemical metabolism.
Gamage et al. (2006)
8 more

17 mentions without citation

The structural biology of oestrogen metabolism.
Thomas et al. (2013)
16 more