› 1hxq

X-ray diffraction
1.86Å resolution

THE STRUCTURE OF NUCLEOTIDYLATED GALACTOSE-1-PHOSPHATE URIDYLYLTRANSFERASE FROM ESCHERICHIA COLI AT 1.86 ANGSTROMS RESOLUTION

Released:
DOI: 10.2210/pdb1hxq/pdb
PDB entry 1hxq coloured by chain, front view.
PDB entry 1hxq coloured by chain, side view.
PDB entry 1hxq coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
The structure of nucleotidylated histidine-166 of galactose-1-phosphate uridylyltransferase provides insight into phosphoryl group transfer.
Wedekind JE, Frey PA, Rayment I
Biochemistry 35 11560-9 (1996)
PMID: 8794735

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-glucose + alpha-D-galactose 1-phosphate = alpha-D-glucose 1-phosphate + UDP-alpha-D-galactose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose-1-phosphate uridylyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 348 amino acids
Theoretical weight: 39.69 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P09148 (Residues: 1-348; Coverage: 100%)
Gene names: JW0741, b0758, galB, galT
Sequence domains:
Structure domains: HIT-like

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand FE 2 x FE
Ligand U5P 2 x U5P
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P21212
Unit cell:
a: 57.2Å b: 215.4Å c: 68.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 not available
Expression system: Escherichia coli

Quick links

Citations

7 review citations

Structure and function of enzymes of the Leloir pathway for galactose metabolism.
Holden et al. (2003)
6 more

4 mentions without citation

Structural classification of zinc fingers: survey and summary.
Krishna et al. (2003)
3 more