› 1hwt

X-ray diffraction
2.5Å resolution

STRUCTURE OF A HAP1/DNA COMPLEX REVEALS DRAMATICALLY ASYMMETRIC DNA BINDING BY A HOMODIMERIC PROTEIN

Released:
DOI: 10.2210/pdb1hwt/pdb
PDB entry 1hwt coloured by chain, front view.
PDB entry 1hwt coloured by chain, side view.
PDB entry 1hwt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
Structure of a HAP1-DNA complex reveals dramatically asymmetric DNA binding by a homodimeric protein.
King DA, Zhang L, Guarente L, Marmorstein R
Nat Struct Biol 6 64-71 (1999)
PMID: 9886294

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113919 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Heme-responsive zinc finger transcription factor HAP1 Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 81 amino acids
Theoretical weight: 9.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CS82 (Residues: 55-135; Coverage: 6%)
Gene names: CYP1, HAP1
Sequence domains: Fungal Zn(2)-Cys(6) binuclear cluster domain
Structure domains:
DNA (5'-D(*GP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*C)-3') Chains: A, E
Molecule details ›
Chains: A, E
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
DNA (5'-D(*GP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*C)-3') Chains: B, F
Molecule details ›
Chains: B, F
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 9 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 85.6Å b: 85.7Å c: 94Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.246 0.246 0.291
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

7 review citations

A fungal family of transcriptional regulators: the zinc cluster proteins.
MacPherson et al. (2006)
6 more

10 mentions without citation

Finding nuclear localization signals.
Cokol et al. (2000)
9 more