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STRUCTURE OF A HAP1/DNA COMPLEX REVEALS DRAMATICALLY ASYMMETRIC DNA BINDING BY A HOMODIMERIC PROTEIN

X-ray diffraction
2.5Å resolution
PDB entry 1hwt coloured by chain, front view.
PDB entry 1hwt coloured by chain, side view.
PDB entry 1hwt coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1
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Assembly Name: Heme-responsive zinc finger transcription factor HAP1 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 15046.79 Å2
Buried surface area: 7224.02 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113919
Assembly 2 (preferred)
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Assembly Name: Heme-responsive zinc finger transcription factor HAP1 and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 14661.78 Å2
Buried surface area: 7227.66 Å2
Dissociation area: 0 Å2
Dissociation energy (ΔGdiss): 0 kcal/mol
Dissociation entropy (TΔSdiss): 0 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113919

Macromolecules

Heme-responsive zinc finger transcription factor HAP1
Chains: C, D, G, H
Length: 81 amino acids
Theoretical weight: 9.63 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CS82 (Residues: 55-135; Coverage: 6%)
Gene names: CYP1, HAP1
Pfam: Fungal Zn(2)-Cys(6) binuclear cluster domain
InterPro:
CATH:
SCOP:
Heme-responsive zinc finger transcription factor HAP1 in PDB entry 1hwt, assembly 2, front view.
Heme-responsive zinc finger transcription factor HAP1 in PDB entry 1hwt, assembly 2, side view.
Heme-responsive zinc finger transcription factor HAP1 in PDB entry 1hwt, assembly 2, top view.
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DNA chains A, E
Name: DNA (5'-D(*GP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*C)-3')
Representative chains: A, E
Source organism: Saccharomyces cerevisiae [4932]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
DNA (5'-D(*GP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*C)-3') in PDB entry 1hwt, assembly 2, front view.
DNA (5'-D(*GP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*C)-3') in PDB entry 1hwt, assembly 2, side view.
DNA (5'-D(*GP*CP*GP*CP*TP*AP*TP*TP*AP*TP*CP*GP*CP*TP*AP*TP*TP*AP*GP*C)-3') in PDB entry 1hwt, assembly 2, top view.
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DNA chains B, F
Name: DNA (5'-D(*GP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*C)-3')
Representative chains: B, F
Source organism: Saccharomyces cerevisiae [4932]
Expression system: Not provided
Length: 20 nucleotides
Theoretical weight: 6.17 KDa
DNA (5'-D(*GP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*C)-3') in PDB entry 1hwt, assembly 2, front view.
DNA (5'-D(*GP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*C)-3') in PDB entry 1hwt, assembly 2, side view.
DNA (5'-D(*GP*CP*TP*AP*AP*TP*AP*GP*CP*GP*AP*TP*AP*AP*TP*AP*GP*CP*GP*C)-3') in PDB entry 1hwt, assembly 2, top view.
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