› 1hu9

X-ray diffraction
2.2Å resolution

LIPOXYGENASE-3 (SOYBEAN) COMPLEX WITH 4-HYDROPEROXY-2-METHOXY-PHENOL

Released:
DOI: 10.2210/pdb1hu9/pdb
PDB entry 1hu9 coloured by chain, front view.
PDB entry 1hu9 coloured by chain, side view.
PDB entry 1hu9 coloured by chain, top view.
Source organism: Glycine max
Primary publication:
Structure of curcumin in complex with lipoxygenase and its significance in cancer.
Skrzypczak-Jankun E, Zhou K, McCabe NP, Selman SH, Jankun J
Int J Mol Med 12 17-24 (2003)
PMID: 12792803

Function and Biology Details

Reaction catalysed: 
Linoleate + O(2) = (9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140587 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Seed linoleate 9S-lipoxygenase-3 Chain: A
Molecule details ›
Chain: A
Length: 857 amino acids
Theoretical weight: 96.92 KDa
Source organism: Glycine max
UniProt:
  • Canonical: P09186 (Residues: 1-857; Coverage: 100%)
Gene names: LOX1.3, LOX3
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand FE 1 x FE
Ligand 4HM 1 x 4HM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 112.79Å b: 137.32Å c: 61.87Å
α: 90° β: 95.55° γ: 90°
R-values:
R R work R free
0.201 0.185 0.271

Quick links

Citations

13 review citations

Curcumin as "Curecumin": from kitchen to clinic.
Goel et al. (2008)
12 more

3 mentions without citation

The structural basis for specificity in lipoxygenase catalysis.
Newcomer et al. (2015)
2 more