› 1ht6

X-ray diffraction
1.5Å resolution

CRYSTAL STRUCTURE AT 1.5A RESOLUTION OF THE BARLEY ALPHA-AMYLASE ISOZYME 1

Released:
DOI: 10.2210/pdb1ht6/pdb
PDB entry 1ht6 coloured by chain, front view.
PDB entry 1ht6 coloured by chain, side view.
PDB entry 1ht6 coloured by chain, top view.
Source organism: Hordeum vulgare
Primary publication:
The structure of barley alpha-amylase isozyme 1 reveals a novel role of domain C in substrate recognition and binding: a pair of sugar tongs.
Robert X, Haser R, Gottschalk TE, Ratajczak F, Driguez H, Svensson B, Aghajari N
Structure 11 973-84 (2003)
PMID: 12906828

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132830 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alpha-amylase type A isozyme Chain: A
Molecule details ›
Chain: A
Length: 405 amino acids
Theoretical weight: 44.64 KDa
Source organism: Hordeum vulgare
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P00693 (Residues: 25-429; Coverage: 98%)
Gene name: AMY1.1
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand EDO 9 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A
Spacegroup: P21212
Unit cell:
a: 88.36Å b: 72.82Å c: 61.74Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.136 0.136 0.163
Expression system: Komagataella pastoris

Quick links

Citations

12 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
11 more

3 mentions without citation

The Role of Aromatic-Aromatic Interactions in Strand-Strand Stabilization of β-Sheets.
Budyak et al. (2013)
2 more