PDBe Entry: 1hqm

CRYSTAL STRUCTURE OF THERMUS AQUATICUS CORE RNA POLYMERASE-INCLUDES COMPLETE STRUCTURE WITH SIDE-CHAINS (EXCEPT FOR DISORDERED REGIONS)-FURTHER REFINED FROM ORIGINAL DEPOSITION-CONTAINS ADDITIONAL SEQUENCE INFORMATION

Summary

Header

TRANSCRIPTION

Method

X-RAY DIFFRACTION

Experiment

Resolution: 3.3 Å, R-factor: 30.0%, Free R-factor: 36.0%, Spacegroup: P 41 21 2

Released

07/02/2001, deposition: 18/12/2000, last revision: 24/02/2009

Authors

Minakhin, L.

; Bhagat, S.

; Brunning, A.

; Campbell, E.A.

; Darst, S.A.

; Ebright, R.H.

; Severinov, K.

Primary citation

Bacterial RNA polymerase subunit omega and eukaryotic RNA polymerase subunit RPB6 are sequence, structural, and functional homologs and promote RNA polymerase assembly.
PROC.NATL.ACAD.SCI.USA

vol:98, pag:892-897 (2001) [PubMed ID 11158566 ]

Keywords

Transferase

, Transcription

, DNA-directed RNA polymerase

, 3D-structure

2.7.7.6 ExPASy BRENDA

(A B C D E)

Organism

Thermus aquaticus 271

(A B C D E)

UniProt

DNA-directed RNA polymerase subunit alpha (EC 2.7.7.6) (RNAP subunit alpha) (Transcriptase subunit alpha) (RNA polymerase subunit alpha) Q9KWU8

(A B)
DNA-directed RNA polymerase subunit beta (EC 2.7.7.6) (RNAP subunit beta) (Transcriptase subunit beta) (RNA polymerase subunit beta) Q9KWU7

(C)
DNA-directed RNA polymerase subunit beta' (EC 2.7.7.6) (RNAP subunit beta') (Transcriptase subunit beta') (RNA polymerase subunit beta') Q9KWU6

(D)
DNA-directed RNA polymerase subunit omega (EC 2.7.7.6) (RNAP omega subunit) (Transcriptase subunit omega) (RNA polymerase omega subunit) Q9EVV4

(E)

Polymers

Id	Name	Type	UniProt	Residues	Observed
A, B	DNA-DIRECTED RNA POLYMERASE	Protein	Q9KWU8 (RPOA_THEAQ)	313	73%
C	DNA-DIRECTED RNA POLYMERASE	Protein	Q9KWU7 (RPOB_THEAQ)	1119	99%
D	DNA-DIRECTED RNA POLYMERASE	Protein	Q9KWU6 (RPOC_THEAQ)	1265	92%
E	DNA-DIRECTED RNA POLYMERASE	Protein	Q9EVV4 (RPOZ_THEAQ)	99	98%

Heterogens

Id	Name	Ligands
D	MAGNESIUM ION	MG
D	ZINC ION	ZN