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EBI PDBe PDBeView

PDBe Entry: 1hpu view

5'-NUCLEOTIDASE (CLOSED FORM), COMPLEX WITH AMPCP
Summary
Header HYDROLASEsearch
Method X-RAY DIFFRACTION
Experiment Resolution: 1.85 Å, R-factor: 20.7%, Free R-factor: 23.4%, Spacegroup: P 1
Released 20/03/2002, deposition: 13/12/2000, last revision: 24/02/2009
Authors Knoefel, T.search; Straeter, N.search
Primary citation Mechanism of hydrolysis of phosphate esters by the dimetal center of 5'-nucleotidase based on crystal structures.
J.MOL.BIOL.search vol:309, pag:239-254 (2001) [PubMed ID 11491293 ]search
Keywords metalloenzymesearch, metallophosphatasesearch, domain rotationsearch, domain movementsearch, HYDROLASEsearch
EC 3.1.3.5 ExPASy BRENDA search 3.6.1.45 ExPASy BRENDA search (A B C D)
Organism Escherichia coli 562search(A B C D)
UniProt Protein ushA precursor [Includes: UDP-sugar hydrolase (EC 3.6.1.45) (UDP-sugar pyrophosphatase) (UDP-sugar diphosphatase); 5'-nucleotidase (EC 3.1.3.5) (5'-NT)] P07024search (A B C D)
Solvent A, B, C, D
Related entries 1ush, 2ush, 1hp1, 1ho5
Polymers
Id Name Type UniProt Residues Observed
A, B, C, D 5'-NUCLEOTIDASE Protein P07024 (USHA_ECOLI)search
 525 100%
Heterogens
Id Name Ligands
A, B, C, D MANGANESE (II) ION MN search
A, B, C, D PHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER A12 search
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