› 1hpm

X-ray diffraction
1.7Å resolution

HOW POTASSIUM AFFECTS THE ACTIVITY OF THE MOLECULAR CHAPERONE HSC70. II. POTASSIUM BINDS SPECIFICALLY IN THE ATPASE ACTIVE SITE

Released:
DOI: 10.2210/pdb1hpm/pdb
PDB entry 1hpm coloured by chain, front view.
PDB entry 1hpm coloured by chain, side view.
PDB entry 1hpm coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
How potassium affects the activity of the molecular chaperone Hsc70. II. Potassium binds specifically in the ATPase active site.
Wilbanks SM, McKay DB
J Biol Chem 270 2251-7 (1995)
PMID: 7836458

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148503 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heat shock cognate 71 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 386 amino acids
Theoretical weight: 42.51 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P19120 (Residues: 1-386; Coverage: 59%)
Gene names: HSC70, HSPA8
Sequence domains: Hsp70 protein
Structure domains:

Ligands and Environments

5 bound ligands:
Ligand MG 1 x MG
Ligand PO4 1 x PO4
Ligand K 2 x K
Ligand CL 2 x CL
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 143.9Å b: 63.7Å c: 46.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 not available
Expression system: Escherichia coli

Quick links

Citations

11 review citations

Interaction of Hsp70 chaperones with substrates.
Rüdiger et al. (1997)
10 more

15 mentions without citation

The nucleotide exchange factors of Hsp70 molecular chaperones.
Bracher et al. (2015)
14 more