› 1hi0

X-ray diffraction
3Å resolution

RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus initiation complex

Released:
DOI: 10.2210/pdb1hi0/pdb
PDB entry 1hi0 coloured by chain, front view.
PDB entry 1hi0 coloured by chain, side view.
PDB entry 1hi0 coloured by chain, top view.
Source organism: Cystovirus phi6
Primary publication:
A mechanism for initiating RNA-dependent RNA polymerization.
Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI
Nature 410 235-40 (2001)
PMID: 11242087

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113899 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
RNA-directed RNA polymerase Chains: P, Q, R
Molecule details ›
Chains: P, Q, R
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:
DNA (5'-(*TP*TP*TP*CP*C)-3') Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 5 nucleotides
Theoretical weight: 1.45 KDa
Source organism: Cystovirus phi6
Expression system: Not provided

Ligands and Environments

3 bound ligands:
Ligand MN 3 x MN
Ligand GTP 6 x GTP
Ligand MG 6 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM14
Spacegroup: P21
Unit cell:
a: 105.65Å b: 92.7Å c: 140.79Å
α: 90° β: 101.09° γ: 90°
R-values:
R R work R free
0.214 0.214 0.239
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

64 review citations

Replication of hepatitis C virus.
Moradpour et al. (2007)
63 more

24 mentions without citation

Common and unique features of viral RNA-dependent polymerases.
te Velthuis AJ. (2014)
23 more