› 1hfu

X-ray diffraction
1.68Å resolution

TYPE-2 CU-DEPLETED LACCASE FROM COPRINUS CINEREUS at 1.68 A resolution

Released:
DOI: 10.2210/pdb1hfu/pdb
PDB entry 1hfu coloured by chain, front view.
PDB entry 1hfu coloured by chain, side view.
PDB entry 1hfu coloured by chain, top view.
Source organism: Coprinopsis cinerea
Primary publication:
Structure of the laccase from Coprinus cinereus at 1.68 A resolution: evidence for different 'type 2 Cu-depleted' isoforms.
Ducros V, Brzozowski AM, Wilson KS, Ostergaard P, Schneider P, Svendson A, Davies GJ
Acta Crystallogr D Biol Crystallogr 57 333-6 (2001)
PMID: 11173497

Function and Biology Details

Reaction catalysed: 
4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195403 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (3 distinct):
Laccase 1 Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 54.41 KDa
Source organism: Coprinopsis cinerea
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: Q9Y780 (Residues: 20-521; Coverage: 96%)
Gene name: lcc1
Sequence domains:
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

Carbohydrate polymer : NEW Components: MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand CU 3 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 45.12Å b: 84.56Å c: 139.03Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.184 0.208
Expression system: Aspergillus oryzae

Quick links

Citations

1 review citation

Biology of Pleurotus eryngii and role in biotechnological processes: a review.
Stajić et al. (2009)
16 other articles

10 mentions without citation

Crystal structure of a blue laccase from Lentinus tigrinus: evidences for intermediates in the molecular oxygen reductive splitting by multicopper oxidases.
Ferraroni et al. (2007)
9 more