spacer NMR STRUCTURE OF THE HUMAN SRC SH2 DOMAIN COMPLEX
Primary citation
Title Solution structure of the human pp60c-src SH2 domain complexed with a phosphorylated tyrosine pentapeptide.
Authors Xu, R.X.search; Word, J.M.search; Davis, D.G.search; Rink, M.J.search; Willard Jr., D.H.search; Gampe Jr., R.T.search
Journal BIOCHEMISTRYsearch vol:34, pag:2107-2121 (1995), Identifiers: PubMed ID (7532003)search DOI (10.1021/bi00007a003)
Abstract Human pp60c-src is a cellular nonreceptor tyrosine kinase that participates in cytosolic signal transduction and has been implicated in the development of malignant tumors in the human breast and colon. Signal transduction is mediated by highly specific interactions between the SH2 domain and receptor phosphorylated tyrosine binding motifs. To elucidate the molecular conformation and interactions in solution, a family of highly resolved nuclear magnetic resonance (NMR) structures was determined for the src SH2 domain complexed with a high-affinity phosphorylated pentapeptide, acetyl-p YEEIE-OH. The 23 structures, generated with a distance geometry (DG) and a dynamical simulated annealing (SA) procedure, satisfied 2072 experimental restraints derived from a variety of multifrequency/multidimensional and isotope-filtered NMR data. Superimposition of residues 143-245 upon the mean coordinate set yielded an atomic rmsd of 0.58 +/- 0.09 A for the N, C alpha, C' atoms and 1.04 +/- 0.08 for all the non-hydrogen atoms. Residues in the ordered secondary structure regions superimpose to 0.29 +/- 0.04 A for the N, C alpha, C' and 0.73 +/- 0.08 A for all the non-hydrogen atoms. The angular order parameter calculated for the phi, psi angles was > 0.9 for 81 of the 106 protein residues. The main protein conformational features are three antiparallel beta-strands that traverse a compact core with an alpha-helix on each side of the core near the N- and C-termini. The observed intermolecular nuclear Overhauser effects (NOE) from the pY, +1E, and +3I residues positioned the ligand in an extended conformation across the SH2 domain surface with the pY and +3I side chains inserted into the protein binding pockets. In general, the protein conformation is consistent with previously reported structures of different SH2 domain complexes determined by X-ray crystallography. However, inter- or intramolecular interactions involving the guanidinium side chains of the solvated R alpha A2 or the buried R beta B5 were not observed at pH = 5.5 or 7.0. If such interactions exist in solution, the absence of any confirming data probably arises from rapid exchange with solvent and/or undetermined dynamic components. Thus, the unrestrained R alpha A2 side chain did not show an amino-aromatic interaction or a hydrogen bond to the -1 carbonyl oxygen as observed in the crystal structures. This result is consistent with the solution structure of a different SH2 domain complex. A more detailed comparison between the crystal structure and the NMR-derived solution structures of the same src SH2 domain complex is presented.(ABSTRACT TRUNCATED AT 400 WORDS)
MeSH terms Amino Acid Sequencesearch, Consensus Sequencesearch, Crystallographysearch, X-Raysearch, Isoenzymessearch, Magnetic Resonance Spectroscopysearch, Modelssearch, Molecularsearch, Molecular Sequence Datasearch, Phospholipase C gammasearch, Phosphotyrosinesearch, Protein Bindingsearch, Protein Structuresearch, Secondarysearch, Protein Structuresearch, Tertiarysearch, Proto-Oncogene Proteins pp60(c-src)search, Signal Transductionsearch, Solutionssearch, Type C Phospholipasessearch, Tyrosinesearch
Other entries described in this publication 1hct
Secondary citations
Title Peptide Inhibitors of Src SH3-Sh2(Slash)Phosphoprotein Interactions
Authors Gilmer, T.search; Rodriguez, M.search; Jordan, S.search; Crosby, R.search; Alligood, K.search; Green, M.search; Kimery, M.search; Wagner, C.search; Kinder, D.search; Charifson, P.search; Hassell, A.M.search; Willard, D.search; Luther, M.search; Rusnak, D.search; Sternbach, D.D.search; Mehrotra, M.search; Peel, M.search; Shampine, L.search; Davis, R.search; Robbins, J.search; Patel, I.R.search; Kassel, D.search; Burkhart, W.search; Moyer, M.search; Bradshaw, T.search; Berman, J.search
Journal J.BIOL.CHEM.search vol:269, pag:31711 (1994)
Title Nuclear Magnetic Resonance Structure of an Sh2 Domain of Phospholipase C-Gamma1 Complexed with a High Affinity Binding Peptide
Authors Pascal, S.M.search; Singer, A.U.search; Gish, G.search; Yamazaki, T.search; Shoelson, S.E.search; Pawson, T.search; Kay, L.E.search; Forman-Kay, J.D.search
Journal CELL(CAMBRIDGE,MASS.)search vol:77, pag:461 (1994)
Title Binding of a High Affinity Phosphotyrosyl Peptide to the Src Sh2 Domain: Crystal Structures of the Complexed and Peptide-Free Forms
Authors Waksman, G.search; Shoelson, S.E.search; Pant, N.search; Cowburn, D.search; Kuriyan, J.search
Journal CELL(CAMBRIDGE,MASS.)search vol:72, pag:779 (1993)
Title Recognition of a High-Affinity Phosphotyrosyl Peptide by the Src Homology-2 Domain of P56Lck
Authors Eck, M.J.search; Shoelson, S.E.search; Harrison, S.C.search
Journal NATUREsearch vol:362, pag:87 (1993)
Title Human Cellular Src Gene: Nucleotide Sequence and Derived Amino Acid Sequence of the Region Coding for the Carboxy-Terminal Two-Thirds of Pp60C-Src
Authors Anderson, S.K.search; Gibbs, C.P.search; Tanaka, A.search; Kung, H.J.search; Fugita, D.J.search
Journal MOL.CELL.BIOL.search vol:5, pag:1122 (1985)
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