› 1h8y

X-ray diffraction
2Å resolution

Crystal structure of the class D beta-lactamase OXA-13 in complex with meropenem

Released:
DOI: 10.2210/pdb1h8y/pdb
PDB entry 1h8y coloured by chain, front view.
PDB entry 1h8y coloured by chain, side view.
PDB entry 1h8y coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Crystal structures of the class D beta-lactamase OXA-13 in the native form and in complex with meropenem.
Pernot L, Frénois F, Rybkine T, L'Hermite G, Petrella S, Delettré J, Jarlier V, Collatz E, Sougakoff W
J Mol Biol 310 859-74 (2001)
PMID: 11453693

Function and Biology Details

Reaction catalysed: 
A beta-lactam + H(2)O = a substituted beta-amino acid
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175930 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Beta-lactamase Chains: A, B
Molecule details ›
Chains: A, B
Length: 247 amino acids
Theoretical weight: 27.45 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q51400 (Residues: 20-266; Coverage: 100%)
Gene name: bla oxa-13
Sequence domains: Penicillin binding protein transpeptidase domain
Structure domains: DD-peptidase/beta-lactamase superfamily

Ligands and Environments

2 bound ligands:
Ligand MER 2 x MER
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 45.34Å b: 112.3Å c: 124.98Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.204 0.257
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Carbapenems: past, present, and future.
Papp-Wallace et al. (2011)
9 more

5 mentions without citation

Three decades of beta-lactamase inhibitors.
Drawz et al. (2010)
4 more