› 1h6r

X-ray diffraction
1.5Å resolution

The oxidized state of a redox sensitive variant of green fluorescent protein

Released:
DOI: 10.2210/pdb1h6r/pdb
PDB entry 1h6r coloured by chain, front view.
PDB entry 1h6r coloured by chain, side view.
PDB entry 1h6r coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Shedding light on disulfide bond formation: engineering a redox switch in green fluorescent protein.
Ostergaard H, Henriksen A, Hansen FG, Winther JR
EMBO J 20 5853-62 (2001)
PMID: 11689426

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 236 amino acids
Theoretical weight: 26.94 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
1 modified residue:
Ligand PIA 3 x PIA

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 57.864Å b: 93.915Å c: 140.551Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.183 0.212
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

81 review citations

Creating new fluorescent probes for cell biology.
Zhang et al. (2002)
80 more

3 mentions without citation

Toward understanding RhoGTPase specificity: structure, function and local activation.
Schaefer et al. (2014)
2 more