› 1h6m

X-ray diffraction
1.64Å resolution

Covalent glycosyl-enzyme intermediate of hen egg white lysozyme

Released:
DOI: 10.2210/pdb1h6m/pdb
PDB entry 1h6m coloured by chain, front view.
PDB entry 1h6m coloured by chain, side view.
PDB entry 1h6m coloured by chain, top view.
Source organism: Gallus gallus
Primary publication:
Catalysis by hen egg-white lysozyme proceeds via a covalent intermediate.
Vocadlo DJ, Davies GJ, Laine R, Withers SG
Nature 412 835-8 (2001)
PMID: 11518970

Function and Biology Details

Reaction catalysed: 
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132831 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Lysozyme C Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.33 KDa
Source organism: Gallus gallus
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: P00698 (Residues: 19-147; Coverage: 100%)
Gene name: LYZ
Sequence domains: C-type lysozyme/alpha-lactalbumin family
Structure domains: Lysozyme

Ligands and Environments

Carbohydrate polymer : NEW Components: G2F, NAG
Carbohydrate polymer
1 bound ligand:
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P43212
Unit cell:
a: 78.632Å b: 78.632Å c: 36.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.172 0.17 0.222
Expression system: Saccharomyces cerevisiae

Quick links

Citations

43 review citations

Glycosyltransferases: structures, functions, and mechanisms.
Lairson et al. (2008)
42 more

5 mentions without citation

Relevant B cell epitopes in allergic disease.
Pomés A. (2010)
4 more