› 1h3b

X-ray diffraction
2.8Å resolution

Function and Biology Details

Reactions catalysed: 
Hopan-22-ol = squalene + H(2)O
Squalene = hop-22(29)-ene
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152568 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Squalene--hopene cyclase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 631 amino acids
Theoretical weight: 71.65 KDa
Source organism: Alicyclobacillus acidocaldarius
Expression system: Escherichia coli K-12
UniProt:
  • Canonical: P33247 (Residues: 1-631; Coverage: 100%)
Gene names: Aaci_2443, shc
Sequence domains:
Structure domains: Glycosyltransferase

Ligands and Environments

2 bound ligands:
Ligand C8E 3 x C8E
Ligand R46 3 x R46
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200B
Spacegroup: P3221
Unit cell:
a: 140.663Å b: 140.663Å c: 243.121Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.206 0.25
Expression system: Escherichia coli K-12

Quick links

Citations

2 review citations

Cholesterol synthesis inhibitor U18666A and the role of sterol metabolism and trafficking in numerous pathophysiological processes.
Cenedella RJ. (2009)
1 more

1 mention without citation

A monodomain class II terpene cyclase assembles complex isoprenoid scaffolds.
Moosmann et al. (2020)