1h27 Summary



The structure was published by Lowe, E.D., Tews, I., Cheng, K.Y., et al., Noble, M.E.M., Gamblin, S., and Johnson, L.N., in 2002 in a paper entitled "Specificity Determinants of Recruitment Peptides Bound to Phospho-Cdk2/Cyclin A" (abstract).

This crystal structure was determined using X-ray diffraction at a resolution of 2.2 Å and deposited in 2002.

The experimental data on which the structure is based was also deposited.

This PDB entry contains a complex of 3 biomacromolecules, namely CELL DIVISION PROTEIN KINASE 2, CYCLIN A2, and CYCLIN-DEPENDENT KINASE INHIBITOR 1B.

The molecule has more than one probable quaternary state observed. For more details see the quaternary structure page.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):

Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 303 98%
C CELL DIVISION PROTEIN KINASE 2 P24941 (1-298) (CDK2_HUMAN)search Homo sapienssearch 97% 303 98%
B CYCLIN A2 P20248 (174-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 259 99%
D CYCLIN A2 P20248 (174-432) (CCNA2_HUMAN)search Homo sapienssearch < 90% 259 99%
E CYCLIN-DEPENDENT KINASE INHIBITOR 1B P46527 (25-35) (CDN1B_HUMAN)search Homo sapienssearch < 90% 11 54%

This entry contains 3 unique UniProt proteins:

UniProt accession Name Organism PDB
P24941 (1 - 298) CELL DIVISION PROTEIN KINASE 2 Homo sapiens
P20248 (174 - 432) CYCLIN A2 Homo sapiens

Chain Structural classification (SCOP) Structural classification (CATH) Sequence family (Pfam)
A, C (P24941) Protein kinases, catalytic subunitsearch Phosphorylase Kinase; domain 1search, Transferase(Phosphotransferase) domain 1search PF00069: Protein kinase domainsearch
B, D Cyclinsearch Cyclin A; domain 1search Cyclin, N-terminal domainsearch, Cyclin, C-terminal domainsearch
E Cyclin-dependent kinase inhibitorsearch

Chain ID Biological process (GO) Molecular function (GO) Cellular component (GO)
A, C (P24941) protein phosphorylationsearch G1/S transition of mitotic cell cyclesearch histone phosphorylationsearch regulation of gene silencingsearch positive regulation of transcription, DNA-templatedsearch meiotic nuclear divisionsearch anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic processsearch centrosome duplicationsearch G2/M transition of mitotic cell cyclesearch mitotic cell cyclesearch potassium ion transportsearch mitotic nuclear divisionsearch phosphorylationsearch positive regulation of cell proliferationsearch DNA replicationsearch cell cyclesearch cell divisionsearch blood coagulationsearch mitotic G1 DNA damage checkpointsearch regulation of ubiquitin-protein ligase activity involved in mitotic cell cyclesearch cellular response to DNA damage stimulussearch DNA repairsearch positive regulation of DNA-dependent DNA replication initiationsearch cellular response to nitric oxidesearch Ras protein signal transductionsearch DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrestsearch mitotic G2 phasesearch ATP bindingsearch protein bindingsearch cyclin bindingsearch cyclin-dependent protein serine/threonine kinase activitysearch kinase activitysearch metal ion bindingsearch protein serine/threonine kinase activitysearch nucleotide bindingsearch transferase activitysearch transferase activity, transferring phosphorus-containing groupssearch protein kinase activitysearch histone kinase activitysearch Y chromosomesearch nucleoplasmsearch nucleussearch cytosolsearch condensed chromosomesearch cytoskeletonsearch cyclin-dependent protein kinase holoenzyme complexsearch cytoplasmsearch Cajal bodysearch endosomesearch microtubule organizing centersearch centrosomesearch transcription factor complexsearch X chromosomesearch chromosome, telomeric regionsearch
B, D (P20248) regulation of cyclin-dependent protein serine/threonine kinase activitysearch regulation of G2/M transition of mitotic cell cyclesearch regulation of cell cyclesearch protein kinase bindingsearch nucleussearch

Chain InterPro annotation
A, C Protein kinase domainsearch Serine/threonine/dual specificity protein kinase, catalytic domainsearch Serine/threonine-protein kinase, active sitesearch Protein kinase-like domainsearch Protein kinase, ATP binding sitesearch
B, D Cyclin, C-terminal domainsearch Cyclin, N-terminalsearch Cyclin-likesearch Cyclin A/B/D/E/Fsearch Cyclin A, chordatessearch