› 1gwl

X-ray diffraction
1.51Å resolution

Carbohydrate binding module family29 complexed with mannohexaose

Released:
DOI: 10.2210/pdb1gwl/pdb
PDB entry 1gwl coloured by chain, front view.
PDB entry 1gwl coloured by chain, side view.
PDB entry 1gwl coloured by chain, top view.
Source organism: Piromyces sp. 'equi'
Primary publication:
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello- and mannohexaose.
Charnock SJ, Bolam DN, Nurizzo D, Szabó L, McKie VA, Gilbert HJ, Davies GJ
Proc Natl Acad Sci U S A 99 14077-82 (2002)
PMID: 12391332

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189899 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
CBM10 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 153 amino acids
Theoretical weight: 17.26 KDa
Source organism: Piromyces sp. 'equi'
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9C171 (Residues: 335-478; Coverage: 31%)
Gene name: ncp1
Structure domains: Galactose-binding lectin

Ligands and Environments

Carbohydrate polymer : NEW Components: BMA
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX9.6
Spacegroup: C2
Unit cell:
a: 51.619Å b: 43.176Å c: 60.731Å
α: 90° β: 93.85° γ: 90°
R-values:
R R work R free
0.156 0.154 0.179
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
9 more

4 mentions without citation

Predicting protein ligand binding sites by combining evolutionary sequence conservation and 3D structure.
Capra et al. (2009)
3 more