› 1gwf

X-ray diffraction
1.96Å resolution

Compound II structure of Micrococcus Lysodeikticus catalase

Released:
DOI: 10.2210/pdb1gwf/pdb
PDB entry 1gwf coloured by chain, front view.
PDB entry 1gwf coloured by chain, side view.
PDB entry 1gwf coloured by chain, top view.
Source organism: Micrococcus luteus
Primary publication:
The structures of Micrococcus lysodeikticus catalase, its ferryl intermediate (compound II) and NADPH complex.
Murshudov GN, Grebenko AI, Brannigan JA, Antson AA, Barynin VV, Dodson GG, Dauter Z, Wilson KS, Melik-Adamyan WR
Acta Crystallogr D Biol Crystallogr 58 1972-82 (2002)
PMID: 12454454

Function and Biology Details

Reaction catalysed: 
2 H(2)O(2) = O(2) + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-151466 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Catalase Chain: A
Molecule details ›
Chain: A
Length: 503 amino acids
Theoretical weight: 57.04 KDa
Source organism: Micrococcus luteus
Expression system: Escherichia coli
UniProt:
  • Canonical: P29422 (Residues: 1-503; Coverage: 100%)
Gene name: katA
Sequence domains:
Structure domains: Catalase core domain

Ligands and Environments


Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
Ligand SO4 3 x SO4
Ligand ACT 2 x ACT
Ligand O 1 x O
1 modified residue:
Ligand SME 5 x SME

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P42212
Unit cell:
a: 106.19Å b: 106.19Å c: 105.3Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.089 0.141 0.19
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Heme enzyme structure and function.
Poulos TL. (2014)
5 more

2 mentions without citation

Monofunctional Heme-Catalases.
Hansberg W. (2022)
1 more