› 1gu8

X-ray diffraction
2.27Å resolution

SENSORY RHODOPSIN II

Released:
DOI: 10.2210/pdb1gu8/pdb
PDB entry 1gu8 coloured by chain, ensemble of 2 models, front view.
PDB entry 1gu8 coloured by chain, ensemble of 2 models, side view.
PDB entry 1gu8 coloured by chain, ensemble of 2 models, top view.
Source organism: Natronomonas pharaonis
Primary publication:
Early structural rearrangements in the photocycle of an integral membrane sensory receptor.
Edman K, Royant A, Nollert P, Maxwell CA, Pebay-Peyroula E, Navarro J, Neutze R, Landau EM
Structure 10 473-82 (2002)
PMID: 11937052

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sensory rhodopsin-2 Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 25.37 KDa
Source organism: Natronomonas pharaonis
Expression system: Escherichia coli
UniProt:
  • Canonical: P42196 (Residues: 1-239; Coverage: 100%)
Gene names: sop2, sopII
Sequence domains: Bacteriorhodopsin-like protein
Structure domains: Rhodopsin 7-helix transmembrane proteins

Ligands and Environments

2 bound ligands:
Ligand RET 1 x RET
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: C2221
Unit cell:
a: 85.51Å b: 128.553Å c: 50.799Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.256
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Halorhodopsin: light-driven ion pumping made simple?
Essen LO. (2002)
3 more

11 mentions without citation

A comprehensive review of the lipid cubic phase or in meso method for crystallizing membrane and soluble proteins and complexes.
Caffrey M. (2015)
10 more