1gru Summary

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SOLUTION STRUCTURE OF GROES-ADP7-GROEL-ATP7 COMPLEX BY CRYO-EM

The structure was published by Ranson, N.A., Farr, G.W., Roseman, A.M., et al., Fenton, W.A., Horwich, A.L., and Saibil, H.R., in 2001 in a paper entitled "ATP-Bound States of Groel Captured by Cryo-Electron Microscopy." (abstract).

The structure was determined using Electron microscopy at a resolution of 12.5 Å and deposited in 2001.

The experimental data on which the structure is based was deposited separately in EMDB as entry 1046.

This PDB entry contains a complex of 2 biomacromolecules, namely GROEL and GROES.

The most likely quaternary structure is a 21meric assembly.

The following tables show cross-reference information to other databases (to obtain a list of all PDB entries sharing the same property or classification, click on the magnifying glass icon):


Chain Name UniProt Name of source organism % of UniProt sequence present in the sample Residues in the sample molecules % of residues observed
A GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
B GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
C GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
D GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
E GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
F GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
G GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
H GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
I GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
J GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
K GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
L GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
M GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
N GROEL P0A6F5 (2-548) (CH60_ECOLI)search Escherichia coli K-12search 96% 547 95%
O GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
P GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
Q GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
R GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
S GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
T GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%
U GROES P0A6F9 (1-97) (CH10_ECOLI)search Escherichia coli K-12search 100% 97 100%


This entry contains 2 unique UniProt proteins:

UniProt accession Name Organism PDB
P0A6F5 (2 - 548) GROEL Escherichia coli
P0A6F9 (1 - 97) GROES Escherichia coli

Chain Structural classification (SCOP) Sequence family (Pfam)
A, B, C, D, E, F, G, H, I, J, K, L, M, N (P0A6F5) GroE chaperonsearch PF00118: TCP-1/cpn60 chaperonin familysearch
O, P, Q, R, S, T, U (P0A6F9) GroE chaperonsearch PF00166: Chaperonin 10 Kd subunitsearch

Chain ID Molecular function (GO) Biological process (GO) Cellular component (GO)
A, B, C, D, E, F, G, H, I, J, K, L, M, N (P0A6F5) ATP bindingsearch protein bindingsearch identical protein bindingsearch ATPase activitysearch unfolded protein bindingsearch nucleotide bindingsearch protein refoldingsearch cellular protein metabolic processsearch cell cyclesearch ATP catabolic processsearch cell divisionsearch virion assemblysearch response to heatsearch protein foldingsearch cytosolsearch cytoplasmsearch membranesearch
O, P, Q, R, S, T, U (P0A6F9) ATP bindingsearch protein bindingsearch identical protein bindingsearch unfolded protein bindingsearch protein foldingsearch virion assemblysearch cell cyclesearch cell divisionsearch response to heatsearch cytoplasmsearch cytosolsearch

Chain InterPro annotation
A, B, C, D, E, F, G, H, I, J, K, L, M, N Chaperonin Cpn60search Chaperonin Cpn60/TCP-1search Chaperonin Cpn60, conserved sitesearch GroEL-like apical domainsearch GroEL-like equatorial domainsearch
O, P, Q, R, S, T, U GroES (chaperonin 10)-likesearch Chaperonin Cpn10, conserved sitesearch Chaperonin Cpn10search