› 1goj

X-ray diffraction
2.3Å resolution

Structure of a fast kinesin: Implications for ATPase mechanism and interactions with microtubules

Released:
DOI: 10.2210/pdb1goj/pdb
PDB entry 1goj coloured by chain, front view.
PDB entry 1goj coloured by chain, side view.
PDB entry 1goj coloured by chain, top view.
Source organism: Neurospora crassa
Primary publication:
Structure of a fast kinesin: implications for ATPase mechanism and interactions with microtubules.
Song YH, Marx A, Müller J, Woehlke G, Schliwa M, Krebs A, Hoenger A, Mandelkow E
EMBO J 20 6213-25 (2001)
PMID: 11707393

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155738 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kinesin heavy chain Chain: A
Molecule details ›
Chain: A
Length: 355 amino acids
Theoretical weight: 38.79 KDa
Source organism: Neurospora crassa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P48467 (Residues: 1-355; Coverage: 38%)
Gene names: NCU09730, kin
Sequence domains: Kinesin motor domain
Structure domains: Kinesin motor domain

Ligands and Environments

2 bound ligands:
Ligand ADP 1 x ADP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P212121
Unit cell:
a: 51.97Å b: 72.73Å c: 84.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 0.264
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

ATPases as drug targets: learning from their structure.
Chène P. (2002)
10 more

9 mentions without citation

Probing the local dynamics of nucleotide-binding pocket coupled to the global dynamics: myosin versus kinesin.
Zheng et al. (2005)
8 more