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CRYSTALLOGRAPHIC ANALYSIS OF THE INTERACTION OF THE GLUCOCORTICOID RECEPTOR WITH DNA

X-ray diffraction
2.9Å resolution
PDB entry 1glu coloured by chain, front view.
PDB entry 1glu coloured by chain, side view.
PDB entry 1glu coloured by chain, top view.
Source organism: Rattus norvegicus
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Glucocorticoid receptor and DNA
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Multimeric state: hetero tetramer
Accessible surface area: 14973.63 Å2
Buried surface area: 5634.73 Å2
Dissociation area: 1,134.68 Å2
Dissociation energy (ΔGdiss): 8.35 kcal/mol
Dissociation entropy (TΔSdiss): 11.34 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-113872

Macromolecules

Glucocorticoid receptor
Chains: A, B
Length: 81 amino acids
Theoretical weight: 9.13 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06536 (Residues: 436-514; Coverage: 10%)
Gene names: Grl, Nr3c1
Pfam: Zinc finger, C4 type (two domains)
InterPro:
CATH: Erythroid Transcription Factor GATA-1, subunit A
SCOP: Nuclear receptor
Glucocorticoid receptor in PDB entry 1glu, assembly 1, front view.
Glucocorticoid receptor in PDB entry 1glu, assembly 1, side view.
Glucocorticoid receptor in PDB entry 1glu, assembly 1, top view.
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DNA chains C, D
Name: DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*GP*AP*TP*GP*TP*TP*C P*TP*G)-3')
Representative chains: C, D
Source organism: Rattus norvegicus [10116]
Expression system: Not provided
Length: 19 nucleotides
Theoretical weight: 5.8 KDa
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*GP*AP*TP*GP*TP*TP*C P*TP*G)-3') in PDB entry 1glu, assembly 1, front view.
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*GP*AP*TP*GP*TP*TP*C P*TP*G)-3') in PDB entry 1glu, assembly 1, side view.
DNA (5'-D(*CP*CP*AP*GP*AP*AP*CP*AP*TP*CP*GP*AP*TP*GP*TP*TP*C P*TP*G)-3') in PDB entry 1glu, assembly 1, top view.
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