› 1gg4

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF ESCHERICHIA COLI UDPMURNAC-TRIPEPTIDE D-ALANYL-D-ALANINE-ADDING ENZYME (MURF) AT 2.3 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1gg4/pdb
PDB entry 1gg4 coloured by chain, front view.
PDB entry 1gg4 coloured by chain, side view.
PDB entry 1gg4 coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of Escherichia coli UDPMurNAc-tripeptide d-alanyl-d-alanine-adding enzyme (MurF) at 2.3 A resolution.
Yan Y, Munshi S, Leiting B, Anderson MS, Chrzas J, Chen Z
J Mol Biol 304 435-45 (2000)
PMID: 11090285

Function and Biology Details

Reaction catalysed: 
ATP + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysine + D-alanyl-D-alanine = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145967 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 452 amino acids
Theoretical weight: 47.91 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11880 (Residues: 1-452; Coverage: 100%)
Gene names: JW0084, b0086, mra, murF
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 18 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-ID
Spacegroup: P61
Unit cell:
a: 74.2Å b: 74.2Å c: 429.3Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.203 0.203 0.28
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

11 review citations

Cytoplasmic steps of peptidoglycan biosynthesis.
Barreteau et al. (2008)
10 more

13 mentions without citation

The Blueprint of a Minimal Cell: MiniBacillus.
Reuß et al. (2016)
12 more