› 1gaj

X-ray diffraction
2.5Å resolution

CRYSTAL STRUCTURE OF A NUCLEOTIDE-FREE ATP-BINDING CASSETTE FROM AN ABC TRANSPORTER

Released:
DOI: 10.2210/pdb1gaj/pdb
PDB entry 1gaj coloured by chain, front view.
PDB entry 1gaj coloured by chain, side view.
PDB entry 1gaj coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structures of the MJ1267 ATP binding cassette reveal an induced-fit effect at the ATPase active site of an ABC transporter.
Karpowich N, Martsinkevich O, Millen L, Yuan YR, Dai PL, MacVey K, Thomas PJ, Hunt JF
Structure 9 571-86 (2001)
PMID: 11470432

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo 24-mer
Assembly name:
PDBe Complex ID:
PDB-CPX-176672 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Probable branched-chain amino acid transport ATP-binding protein LivG Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.03 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58663 (Residues: 1-257; Coverage: 100%)
Gene names: MJ1267, livG
Sequence domains: ABC transporter
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand SO4 1 x SO4
Ligand CL 2 x CL
Ligand TBU 3 x TBU
Ligand PEG 2 x PEG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12B
Spacegroup: P432
Unit cell:
a: 137.52Å b: 137.52Å c: 137.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.205 0.243
Expression system: Escherichia coli

Quick links

Citations

59 review citations

Siderophore-based iron acquisition and pathogen control.
Miethke et al. (2007)
58 more

2 mentions without citation

Mechanism of ABC transporters: a molecular dynamics simulation of a well characterized nucleotide-binding subunit.
Jones et al. (2002)
1 more