› 1g9s

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF A COMPLEX BETWEEN E.COLI HPRT AND IMP

Released:
DOI: 10.2210/pdb1g9s/pdb
PDB entry 1g9s coloured by chain, front view.
PDB entry 1g9s coloured by chain, side view.
PDB entry 1g9s coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase.
Guddat LW, Vos S, Martin JL, Keough DT, de Jersey J
Protein Sci 11 1626-38 (2002)
PMID: 12070315

Function and Biology Details

Reaction catalysed: 
IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141900 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypoxanthine phosphoribosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.66 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A9M2 (Residues: 1-178; Coverage: 100%)
Gene names: JW5009, b0125, hpt
Sequence domains: Phosphoribosyl transferase domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand IMP 1 x IMP
Ligand N 1 x N
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 14-BM-D
Spacegroup: P3121
Unit cell:
a: 84.14Å b: 84.14Å c: 167.27Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.243
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Direct binding targets of the stringent response alarmone (p)ppGpp.
Kanjee et al. (2012)
20 other articles

1 mention without citation

Crystal structures of free, IMP-, and GMP-bound Escherichia coli hypoxanthine phosphoribosyltransferase.
Guddat et al. (2002)