› 1g8i

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF HUMAN FREQUENIN (NEURONAL CALCIUM SENSOR 1)

Released:
DOI: 10.2210/pdb1g8i/pdb
PDB entry 1g8i coloured by chain, front view.
PDB entry 1g8i coloured by chain, side view.
PDB entry 1g8i coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Immunocytochemical localization and crystal structure of human frequenin (neuronal calcium sensor 1).
Bourne Y, Dannenberg J, Pollmann V, Marchot P, Pongs O
J Biol Chem 276 11949-55 (2001)
PMID: 11092894

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158585 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neuronal calcium sensor 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 190 amino acids
Theoretical weight: 21.9 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P62166 (Residues: 1-190; Coverage: 100%)
Gene names: FLUP, FREQ, NCS1
Sequence domains:
Structure domains: EF-hand

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand CA 6 x CA
Ligand 1PE 3 x 1PE
Ligand P6G 1 x P6G
Ligand EDO 2 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21
Unit cell:
a: 53.811Å b: 55.524Å c: 77.705Å
α: 90° β: 94.25° γ: 90°
R-values:
R R work R free
0.22 0.22 0.263
Expression system: Escherichia coli BL21

Quick links

Citations

23 review citations

Structural basis for diversity of the EF-hand calcium-binding proteins.
Grabarek Z. (2006)
22 more

16 mentions without citation

Neuronal calcium sensor proteins: generating diversity in neuronal Ca2+ signalling.
Burgoyne RD. (2007)
15 more