› 1g4p

X-ray diffraction
2.5Å resolution

Function and Biology Details

Reaction catalysed: 
4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamine-phosphate synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 226 amino acids
Theoretical weight: 24.16 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: P39594 (Residues: 1-222; Coverage: 100%)
Gene names: BSU38290, ipa-26d, thiC, thiE, ywbK
Sequence domains: Thiamine monophosphate synthase
Structure domains: Aldolase class I

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand FQP 2 x FQP
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P43212
Unit cell:
a: 76.28Å b: 76.28Å c: 139.59Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.219 0.219 0.295
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Radical S-adenosylmethionine enzymes.
Broderick et al. (2014)
4 more

2 mentions without citation

Identification of inhibitors against Mycobacterium tuberculosis thiamin phosphate synthase, an important target for the development of anti-TB drugs.
Khare et al. (2011)
1 more