› 1g3n

X-ray diffraction
2.9Å resolution

STRUCTURE OF A P18(INK4C)-CDK6-K-CYCLIN TERNARY COMPLEX

Released:
DOI: 10.2210/pdb1g3n/pdb
PDB entry 1g3n coloured by chain, front view.
PDB entry 1g3n coloured by chain, side view.
PDB entry 1g3n coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis of inhibition of CDK-cyclin complexes by INK4 inhibitors.
Jeffrey PD, Tong L, Pavletich NP
Genes Dev 14 3115-25 (2000)
PMID: 11124804

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero tetramer
PDBe Complex ID:
PDB-CPX-154869 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Cyclin-dependent kinase 6 Chains: A, E
Molecule details ›
Chains: A, E
Length: 326 amino acids
Theoretical weight: 36.99 KDa
Source organism: Homo sapiens
Expression system: unidentified baculovirus
UniProt:
  • Canonical: Q00534 (Residues: 1-326; Coverage: 100%)
Gene names: CDK6, CDKN6
Sequence domains: Protein kinase domain
Structure domains:
Cyclin-dependent kinase 4 inhibitor C Chains: B, F
Molecule details ›
Chains: B, F
Length: 168 amino acids
Theoretical weight: 18.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P42773 (Residues: 1-168; Coverage: 100%)
Gene names: CDKN2C, CDKN6
Sequence domains: Ankyrin repeats (3 copies)
Structure domains: Ankyrin repeat-containing domain
Cyclin-like domain-containing protein Chains: C, G
Molecule details ›
Chains: C, G
Length: 257 amino acids
Theoretical weight: 28.09 KDa
Source organism: Human gammaherpesvirus 8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98147 (Residues: 1-257; Coverage: 100%)
Sequence domains:
Structure domains: Cyclin-like

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 77.78Å b: 146.95Å c: 164.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.242 0.224 0.262
Expression systems:
  • unidentified baculovirus
  • Escherichia coli

Quick links

Citations

36 review citations

The history and future of targeting cyclin-dependent kinases in cancer therapy.
Asghar et al. (2015)
35 more

34 mentions without citation

A primer on ankyrin repeat function in TRP channels and beyond.
Gaudet R. (2008)
33 more